![]() The DL2000 DNA Marker (TaKaRa) was used as a size standard (Ms). (B) Genomic PCR shows that PCc pab-1 (up) and TCc pab-1 (down) from pCc pab-1 and PAb gpdII (up) and TAn trpC (down) from pCcExp in the mock transformant, PCc pab-1 (up) and TCc pab-1 (down) from pCc pab-1, PAb gpdII- nsdD1antisense (up) and nsdD1sense-TAn trpC (down) from pCc nsdD1dsRNA, and PAb gpdII- nsdD2antisense (up) and nsdD2sense-TAn trpC (down) from pCc nsdD2dsRNA in the representative CcNsdD1/NsdD2-RNAi transformants (T2, T12, T43, and T58), PCc pab-1 (up) and TCc pab-1 (down) from pCc pab-1, and PAb gpdII- nsdD2 (up) and nsdD2-TAn trpC (down) from pCc nsdD2OE in the representative CcNsdD2-OE transformants (T3, T5, T12, and T21), were integrated into the genome. The arrows and lines below the plasmids indicate the primers for genomic PCR and the hybridization probes for Southern blotting, respectively. (A) Schematic representation of plasmids pCc pab-1, pCcExp, pCc nsdD1dsRNA, pCc nsdD2dsRNA, pCc nsdD1OE, and pCc nsdD2OE. In addition, the result that overexpression of CcnsdD2 induced more secondary hyphal knots, primordia, and fruiting bodies under light/dark rhythm cultivation conditions has potential applied value in the edible mushroom industry.Ĭoprinopsis cinerea fruiting body light stimulation primary hyphal knot primordium sclerotium secondary hyphal knot transcription factor. This study provides new insight into the molecular mechanism by which CcNsdD2 regulates the developmental fate of C. Although the induction of CcnsdD2 is not under direct control of light and photoreceptors, the CcNsdD2-mediated developmental fates of the primary hyphal knots depend on the following light/dark rhythm cultivation or dark cultivation after full growth of mycelia in the constant dark cultivation. cinerea transcription factor CcNsdD2 promotes primary hyphal knot formation by upregulating cfs1, cfs2, cgl1, and hyd1. IMPORTANCE The model mushroom Coprinopsis cinerea exhibits remarkable photomorphogenesis during fruiting body development. cinerea under dark or light conditions is proposed. A molecular mechanism by which CcNsdD2 regulates the developmental fate of C. ChIP-seq and electrophoretic mobility shift assay demonstrated that CcNsdD2 bound to promoter regulatory sequences containing a GATC motif in cfs1, cfs2, cgl1, and hyd1. RNA-seq revealed that some genes reported previously to be involved in formation of hyphal knots and primordia, cyclopropane-fatty-acyl-phospholipid synthases cfs1-3, galectins cgl1-3, and hydrophobins hyd1-3 were downregulated in the CcNsdD1/NsdD2-RNAi transformant compared to the mock transformant. The CcNsdD2-overexpressing transformant produced more primary hyphal knots, secondary hyphal knots, and fruiting bodies under a light/dark rhythm but only more primary hyphal knots and sclerotia under darkness. Knockdown of CcnsdD1/ nsdD2 led to the differentiation of primary hyphal knots into sclerotia rather than secondary hyphal knots under a light/dark rhythm, similar to the differentiation of primary hyphal knots into sclerotia of the wild-type strain under darkness. Although single knockdown of CcnsdD2 did not affect fruiting body production due to upregulation of its homolog CcnsdD1, the double-knockdown CcNsdD1/NsdD2-RNAi transformant showed defects in fruiting body formation under a light/dark rhythm. After 4 days of constant darkness cultivation, CcnsdD2, but not CcnsdD1, was upregulated on the first day of light/dark cultivation to induce fruiting bodies, and overexpression of CcnsdD2, but not CcnsdD1, produced more fruiting bodies under a light/dark rhythm. cinerea show the best identities of 62 and 50% to A. Coprinopsis cinerea has seven homologs of the Aspergillus nidulans transcription factor NsdD. ![]()
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